Amino acid dating forensic science

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  1. Proteomics
  2. Amino acid dating
  3. Age Estimation in Forensic Sciences
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  5. Age Estimation in Forensic Sciences

If the sex of a person is unknown, the average time for enamel completion for males and females is calculated. The 14 C concentration measured in the tooth enamel is plotted onto a curve of atmospheric 14 C against time to determine the year of enamel synthesis and date of birth of the individual.

The time in years taken for the enamel to form is subtracted from the year obtained to give an estimated date of birth Fig. Calibrated ages were obtained by using the CALIbomb Levin data set where the smoothing in years was set at 1. In CALIbomb, these values are predicted using a straight line from prebomb values to For these age ranges, values from the Hua and Barbetti 26 were used.

Application of combined analysis of radiocarbon and aspartic acid racemization on four teeth collected from homicide case. Three teeth with relatively short enamel laydown times showed no bomb spike-derived 14 C in the enamel. However, the fourth tooth, an upper jaw third molar with a much longer enamel formation period, did and matched atmospheric 14 C levels equating to This indicates a birth date of By adding the average age red dashed line estimated from the racemization analysis, the year of death was calculated red vertical line to be DOB , date of birth.

If it is not obvious whether an individual is born before or after the peak of the bomb tests, then two teeth with different enamel laydown times need to be analyzed; this will distinguish whether the 14 C measurement relates to the rising or falling part of the curve 4. Additional studies by Cook et al.

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Because determining which side of the bomb peak a person falls has previously been demonstrated, we do not include the analysis of two teeth for this purpose here but have rather chosen the age range appropriate to the known date of birth of the individual. In the summer of , hunters found a human skeleton covered by a tarpaulin in a Swedish forest.

Forensic examination revealed that the person had been shot in the back of his skull. Although the skeleton was almost complete, the consultant forensic anthropologist could not determine the deceased age with any precision but stated that it was a male and suggested he had been about 30—40 years old at the time of death. The anthropologist further estimated that the body had been dead for less than 15 years.

Four teeth were extracted and subjected to both 14 C analysis and aspartic acid racemization as described previously. Teeth from individuals with dates of birth ranging from through aged 13—70 years were analyzed see supplemental Table 1.

Amino acid dating

Regression analysis of date of birth estimations using radiocarbon dating of tooth enamel and aspartic acid racemization analysis of crown dentin reveal a strong correlation between the two methods for teeth formed during and after the period of bomb testing Fig. Although both methods are in good agreement with each other, radiocarbon dating offered more precise age estimations than aspartic acid racemization analysis. Radiocarbon analysis of tooth enamel from nine Swedish individuals 10 teeth , where the date of birth was known, yielded a 14 C-based date of birth estimation accuracy of 1.

Two teeth that were formed prior to the period of nuclear weapon testing showed prebomb radiocarbon values. Aspartic acid racemization analysis of crown dentin using the same teeth yielded an average error of age estimation of 6. Teeth from an additional 22 individuals were also analyzed: As in the first round, radiocarbon dating gave a more precise estimate of chronological age than aspartic acid racemization analysis radiocarbon dating age estimate precision was 0.

Associated Data

An analysis of all cases demonstrated the average age estimate precision of radiocarbon dating to be 1. Teeth from the same number of individuals were studied for each method; however, three individuals analyzed for radiocarbon dating showed prebomb levels of radiocarbon in their enamel. As such, one can only conclude that the said individual is born at least x number of years before the beginning of the bomb pulse where x indicates the enamel laydown time. In all cases where the analyzed tooth was formed before the period of nuclear bomb testing, AMS readings indicated prebomb radiocarbon values.

For details regarding each case, see Table I and supplemental Table 1. The two 14 C cases giving prebomb values are omitted.

Age Estimation in Forensic Sciences

Analysis of the whole crushed crown as opposed to purified enamel also results in precise date of birth estimation Table II. Crushing the complete tooth crown significantly simplifies the processing needed for radiocarbon analysis and cuts the preprocessing time down to 15 min. Four teeth from an ongoing homicide case in Sweden were analyzed for enamel radiocarbon content as well as aspartic acid racemization of crown dentin.

Radiocarbon analysis showed that three of the teeth, with enamel laydown times ranging from 2. One tooth, a third molar from the upper jaw tooth number 48 , did contain bomb carbon in the enamel. Given that the enamel laydown time for this tooth is 13 years, the victim's date of birth was estimated at Because no exact date of death is known for this case, it is not possible using radiocarbon analysis alone to determine the chronological age of the victim.

Aspartic acid racemization analysis of crown dentin from the same four teeth indicated that the victim was Combining the age of the victim with the date of birth, it is possible to estimate the date of death as being The resistance of teeth to environmental influence makes them particularly valuable in the forensic setting.

Teeth can remain intact for an appreciable time and allow for an odontologic identification of even very old skeletons provided ante-mortem data are available. Furthermore, no exchange of carbon will take place in the enamel of the mature tooth during life and generally not after death, making mature permanent teeth particularly suitable for radiocarbon analysis.

We show that both aspartic acid racemization and AMS radiocarbon analyses can be performed on a single tooth and that the combined analysis can provide information about the year of birth and year of death of an individual. Both methods correlate well. In this study, we analyzed almost double the number of individuals reported in the first study and made minor adjustments to the methods.

We now report an average absolute error of date of birth estimation of 1. Aspartic acid racemization analysis of dentin also allowed for a fair prediction of the age of the person with a precision comparable to that reported previously for a review, see Ref. Both methods have strengths and limitations. The radiocarbon birth dating method can tell the birth date of the person regardless of the time of death. However, the time window for this analysis is limited to subjects born after the early s because the calculations are based on the measurement of bomb pulse-derived 14 C.

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For older subjects, analysis of third molars if available may be necessary to detect bomb carbon because of the delay in their enamel laydown time. This is well illustrated by the described homicide case. Four teeth were analyzed for enamel radiocarbon content with enamel laydown time ranging from 2. Although no radiocarbon could be detected in the three teeth with the shortest enamel laydown period 2.

From this, we could deduce that the victim was born in Aspartic acid racemization analysis of the four teeth estimated the victim to be Despite Interpol contacts, this person was never identified. The police do have an idea of who this person might be, a foreigner believed to be in his forties who was suspected for having set fire to a restaurant in but then disappeared. The results of the radiocarbon and aspartic acid racemization analyses provide additional support for this theory and illustrate how combining both methodologies can provide precise and valuable information to assist police authorities.

Aspartic acid racemization analysis of dentin provides information about the chronological age of an individual at death because the chemical conversion from the l -enantiomer to the d -enantiomer will typically stop completely after death. Thus, analysis is independent of the calendar years in which the person was born and died. To determine these calendar years, other methods must be used, and usually the only alternative is to estimate the post-mortem interval. The wide window for this interval given by the experienced anthropologist in the homicide case serves as an example of the difficulties this often poses.

One advantage of aspartic acid racemization analysis is that it is independent of the bomb spike and hence can be used for age determination of subjects born long before the beginning of aboveground nuclear weapon testing. Several factors, however, will affect the precision of this method.


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Because the racemization process is basically a function of temperature and time, teeth are exposed to different ambient temperatures depending on their location. They suggest that in elderly individuals racemization in teeth that have been situated deep in the oral cavity for a long time and thus are exposed to higher ambient temperatures are more influenced by the environment than by the period of tooth formation.

Amino acid specific reagent for detection of fingerprints (FSC)

Selection of which tooth to analyze may therefore yield different results depending on the age of the individual. The types of teeth best suited for racemization analysis are single rooted teeth such as mandibular incisors or mandibular premolars 13 , In these teeth, all the dentin can be easily collected, and test results have shown that analysis using whole dentin yields a more accurate age estimate than analysis using only part of the dentin The age estimation precision reported here for aspartic acid racemization of dental enamel was conducted on an assortment of teeth and would most likely have been significantly higher if mandibular single rooted teeth had been available and used for analysis.

In addition, some of the teeth were collected from teenagers where complete root formation may not have been completed. In such cases, it is impossible to collect whole dentin, resulting in poorer age estimation as described above. This means that the amino acid can have two different configurations, "D" or "L" which are mirror images of each other.

With a few important exceptions, living organisms keep all their amino acids in the "L" configuration. When an organism dies, control over the configuration of the amino acids ceases, and the ratio of D to L moves from a value near 0 towards an equilibrium value near 1, a process called racemization. Thus, measuring the ratio of D to L in a sample enables one to estimate how long ago the specimen died.

The rate at which racemization proceeds depends on the type of amino acid and on the average temperature, humidity, acidity pH , and other characteristics of the enclosing matrix. Temperature and humidity histories of microenvironments are being produced at ever increasing rates as technologies advance and technologists accumulate data.

These are important for amino acid dating because racemization occurs much faster in warm, wet conditions compared to cold, dry conditions. Temperate to cold region studies are much more common than tropical studies, and the steady cold of the ocean floor or the dry interior of bones and shells have contributed most to the accumulation of racemization dating data. Strong acidity and mild to strong alkalinity induce greatly increased racemization rates. Generally, they are not assumed to have a great impact in the natural environment, though tephrochronological data may shed new light on this variable.

The enclosing matrix is probably the most difficult variable in amino acid dating. This includes racemization rate variation among species and organs, and is affected by the depth of decomposition, porosity, and catalytic effects of local metals and minerals. This amino acid ratio has the advantages of being relatively easy to measure and being chronologically useful through the Quaternary. Reverse phase HPLC techniques can measure up to 9 amino acids useful in geochronology over different time scales on a single chromatogram aspartic acid , glutamic acid , serine , alanine , arginine , tyrosine , valine , phenylalanine , leucine.

In recent years there have been successful efforts to examine intra-crystalline amino acids separately as they have been shown to improve results in some cases. Data from the geochronological analysis of amino acid racemization has been building for thirty-five years.

Archeology , [13] stratigraphy , oceanography , paleogeography , paleobiology , and paleoclimatology have been particularly affected.

Age Estimation in Forensic Sciences

Their applications include dating correlation, relative dating, sedimentation rate analysis, sediment transport studies, [14] conservation paleobiology, [15] taphonomy and time-averaging, [16] [17] [18] sea level determinations, and thermal history reconstructions. Paleobiology and archaeology have also been strongly affected.

Bone, shell, and sediment studies have contributed much to the paleontological record, including that relating to hominoids. Verification of radiocarbon and other dating techniques by amino acid racemization and vice versa has occurred. L- and D- forms enantiomers. Enantiomers are mirror images that are not superimposable- like the right and left hand.

Only the L-forms are used by cells. When humans age, L-forms amino acids will transform into D-forms via the process racemization. So we can make use of this fact to estimate age. Furthermore, tissues with lower metabolic rates would be better used for age estimation due to the continuous synthesizing and degradation of amino acids in the human body.


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Known more commonly as Carbon dating. Radiocarbon dating of the tooth enamel provided high precision to determine the date of birth of an individual. The concept of half-live is used. A human skeleton was discovered in a Swedish forest in summer They extracted 4 teeth from the skeleton. Dentin was used for the racemization of aspartic acid.